Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/PR
From 2013.igem.org
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'''1. Goals for the week''' | '''1. Goals for the week''' | ||
: Our goals for the past few weeks were to establish that the basic procedures for our direct evolution method works. Specifically, we can grow T7 in liquid culture with E. coli, amplify phage to create a high titer, select for smaller phage using concentrated top agar, and propagate phage from a single plaque. | : Our goals for the past few weeks were to establish that the basic procedures for our direct evolution method works. Specifically, we can grow T7 in liquid culture with E. coli, amplify phage to create a high titer, select for smaller phage using concentrated top agar, and propagate phage from a single plaque. | ||
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:: However, concentrated top agar solidify so fast that we weren’t able to get it even spread on the plate. We decided to try warming up the plates in 37°C incubator for 3 hours before use (5.9 T7 Phage Selection Method Test #2). Our plates are much prettier this time. | :: However, concentrated top agar solidify so fast that we weren’t able to get it even spread on the plate. We decided to try warming up the plates in 37°C incubator for 3 hours before use (5.9 T7 Phage Selection Method Test #2). Our plates are much prettier this time. | ||
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: We have designed primer for amplifying and sequencing T7 capsid gene. | : We have designed primer for amplifying and sequencing T7 capsid gene. | ||
: We have also designed detailed procedure for applying mutagen and PCR. | : We have also designed detailed procedure for applying mutagen and PCR. | ||
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'''3. Next Steps''' | '''3. Next Steps''' | ||
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Revision as of 17:08, 27 May 2013
Small Phage Spring Notebook: April 29 - May 12 Progress Report
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Overview
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1. Goals for the week
2.Experiments and results Phage growth in liquid culture (5.3 Phage Amplification/Purification)
Amplifying phage (5.6 T7+ Liquid Culture Phage Concentration Test)
Selection test
Stabbing test (5.5 Amplification from a plaque test)
Others
3. Next Steps
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