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Exeter/12 August 2013
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(Created page with "Mini Preps of liquid cultures that worked ==NanoDrop== {| class="wikitable" |- ! Culture !! NanoDrop concentration (ng/ul) !! ul to get 250ng |- | AMP plasmid || 60.0 || 4.17...")
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(Created page with "Mini Preps of liquid cultures that worked ==NanoDrop== {| class="wikitable" |- ! Culture !! NanoDrop concentration (ng/ul) !! ul to get 250ng |- | AMP plasmid || 60.0 || 4.17...")
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Revision as of 12:07, 15 August 2013
Mini Preps of liquid cultures that worked
NanoDrop
| Culture | NanoDrop concentration (ng/ul) | ul to get 250ng |
|---|---|---|
| AMP plasmid | 60.0 | 4.17 |
| Yellow (K592010) | 49.1 | 5.09 |
| RBS + cph8 | 81.2 | 3.08 |
| Terminators | 27.9 | 8.96 |
| RFP control | 51.3 | 4.87 |
| RBS + yellow (S05058) | 56.7 | 4.41 |
| OmpF | 76.7 | 3.20 |
| Promoter + RBS | 62.6 | 3.99 |
| Magenta | 368.1 | 0.67 |
| Magenta diluted | 35.7 | 7.0 |
| Culture | ul to get 250ng | 16ul - answer |
|---|---|---|
| AMP plasmid | 4.17 | 11.83 |
| Yellow (K592010) | 5.09 | 10.91 |
| B0015 | 8.96 | 7.04 |
| RFP | 4.87 | 11.13 |
| RBS + yellow (S05058) | 4.41 | 11.59 |
| Magenta diluted | 7.0 | 9.0 |
