Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Explist

From 2013.igem.org

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<font size="4"> '''5.3 T7 phage selection method test''' </font>
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<font size="4"> '''5.3 T7 Phage Selection Method Test''' </font>
In this experiment, we aimed to test whether or not we can select for smaller phages using concentrated top agar.
In this experiment, we aimed to test whether or not we can select for smaller phages using concentrated top agar.
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 phage selection method test]]
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 Phage Selection Method Test]]
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<br>
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<font size="4"> '''5.3 T7 phage amplification/purification''' </font>
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<font size="4"> '''5.3 T7 Phage Amplification/Purification''' </font>
In this experiment, we aimed to establish a basic protocol for amplifying and purifying phage.  
In this experiment, we aimed to establish a basic protocol for amplifying and purifying phage.  
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Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]]
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Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.3 Phage Amplification/Purification|5.3 T7 Phage Amplification/Purification]]
<br>
<br>
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<font size="4"> '''5.5 Amplification From a Plaque Test''' </font>
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In this experiment, we aimed to test whether or not we can amplify a phage plaque using liquid culture
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.5_Amplification_from_a_plaque_test|5.5 Amplification From a Plaque Test]]
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<font size="4"> '''5.6 T7+ Liquid Culture Phage Concentration Test ''' </font>
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In this experiment, we aimed to test whether or not starting phage liquid culture with different concentrations of phage will affect the overall yield of phage progeny.
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.6_T7%2B_Liquid_Culture_Phage_Concentration_Test|5.6 T7+ Liquid Culture Phage Concentration Test ]]
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<font size="4"> '''5.9 T7 Selection Method Test #2''' </font>
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We performed this experiment to familiarize ourselves with x8 agar.
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/5.9_T7_selection_method_test|5.9 T7 Selection Method Test #2]]
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<br>
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<font size="4"> '''5.9 T7+ Liquid Culture Phage Concentration Test #2''' </font>
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We repeated [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.6_T7%2B_Liquid_Culture_Phage_Concentration_Test|5.6 T7+ Liquid Culture Phage Concentration Test ]] to test for whether 1uL will give us even higher phage titer
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Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/5.9 T7+ Liquid Culture Phage Concentration Test|5.9 T7+ Liquid Culture Phage Concentration Test #2]]
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Revision as of 20:26, 27 May 2013


Small Phage Spring Notebook: April 29 - May 12 Experiment Listing



Overview
Winter
Spring
Summer
Fall

5.3 T7 Phage Selection Method Test

In this experiment, we aimed to test whether or not we can select for smaller phages using concentrated top agar.

Detailed procedure: 5.3 T7 Phage Selection Method Test


5.3 T7 Phage Amplification/Purification

In this experiment, we aimed to establish a basic protocol for amplifying and purifying phage.

Detailed procedure: 5.3 T7 Phage Amplification/Purification


5.5 Amplification From a Plaque Test

In this experiment, we aimed to test whether or not we can amplify a phage plaque using liquid culture

Detailed procedure: 5.5 Amplification From a Plaque Test


5.6 T7+ Liquid Culture Phage Concentration Test

In this experiment, we aimed to test whether or not starting phage liquid culture with different concentrations of phage will affect the overall yield of phage progeny.

Detailed procedure: 5.6 T7+ Liquid Culture Phage Concentration Test


5.9 T7 Selection Method Test #2

We performed this experiment to familiarize ourselves with x8 agar.

Detailed procedure: 5.9 T7 Selection Method Test #2


5.9 T7+ Liquid Culture Phage Concentration Test #2

We repeated 5.6 T7+ Liquid Culture Phage Concentration Test to test for whether 1uL will give us even higher phage titer

Detailed procedure: 5.9 T7+ Liquid Culture Phage Concentration Test #2


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