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Team:Goettingen/NoteBook w1
From 2013.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
<html> | <html> | ||
| + | <h2>June 6th, Thursday</h2> | ||
| + | <p class="timeline-title">Pick the colonies of part1-7</p> | ||
| + | <b>Media preparation</b> | ||
| + | <p>1000ml LB+Amp Solid medium => about 50 Plates(with black code)</p> | ||
| + | <b>Transformation</b> | ||
| + | <table cellspacing="0"> | ||
| + | <tr><th>Entry Numer</th><th>Location on the kit</th> | ||
| + | <tr><td>BBa_B0034</td><td>P5 2 M</td> | ||
| + | <b>Pick the colonies of Part1-7</b> | ||
| + | 4ml LB with antibiotics, overnight culture for mini-prep[marked with C1]<br /> | ||
| + | Spread backup plates:marked with C1,C2,C3 for each part. | ||
| + | |||
| + | |||
<h2>June 5th, Wednesday</h2> | <h2>June 5th, Wednesday</h2> | ||
| - | <p class="timeline-title"> | + | <p class="timeline-title">Preparation of the medium, antibioticks, Transformation.</p> |
<b>Preparation of Antibiotic Stocks</b> | <b>Preparation of Antibiotic Stocks</b> | ||
<ul> | <ul> | ||
| Line 35: | Line 48: | ||
<h2>June 4th, Tuesday</h2> | <h2>June 4th, Tuesday</h2> | ||
| - | <p class="timeline-title"> | + | <p class="timeline-title">Find the correct DNA sequence of DarR and primer design</p> |
<table cellspacing="0"> | <table cellspacing="0"> | ||
<tr style="background = #eee"><th>Code Name</th><th> Description</th></tr> | <tr style="background = #eee"><th>Code Name</th><th> Description</th></tr> | ||
Revision as of 05:50, 17 August 2013
June 6th, Thursday
Pick the colonies of part1-7
Media preparation1000ml LB+Amp Solid medium => about 50 Plates(with black code)
Transformation| Entry Numer | Location on the kit | |||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BBa_B0034 | P5 2 M | Pick the colonies of Part1-7 4ml LB with antibiotics, overnight culture for mini-prep[marked with C1]
| Code Name | Description |
|---|---|
| iGEM_36 | DarR operator sequence + biobrick prefix |
| iGEM_37 | DarR operator sequence + biobrick sufix |
Transformation
| Biobrick entry number | Mark |
|---|---|
| BBa_J23117 | part 1 |
| BBa_J23116 | part 2 |
| BBa_J23110 | part 3 |
| BBa_J23118 | part 4 |
| BBa_J61101 | part 5 |
| BBa_BE0240-Cm | part 6 -- Cm |
| BBa_B0015 | part 7 -- Cm |
| BBa_B0034 | part 8 |
| Resuspended but not transformed | |
| Entry number | location on kit |
| BBa_E0204-Amp | P5 12 M |
| BBa_QO3121 | P5 20 N |
June 4th, Tuesday
Find the correct DNA sequence of DarR and primer design
| Code Name | Description |
|---|---|
| iGEM_32 | Forward primer for DarR ORF amplification, with biobrick prefix |
| iGEM_33 | Reverse primer for DarR ORF amplification, with biobrick sufix |
| iGEM_34 | Forward primer for DarR ORF PCR amplification sequencing. |
| iGEM_35 | Reverse primer for DarR ORF PCR amplification sequencing. |
