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20/08/13
From 2013.igem.org
(Difference between revisions)
(Created page with "==Making nutrient agar plates and broth== *Making up broth and agar solution to grow ''P. putida'' in. *For agar plates: **9.2g of nutrient agar medium **400ml of distilled water...") |
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*Freeze dried ''P. putida'' was rehydrated using 1ml of nutrient broth | *Freeze dried ''P. putida'' was rehydrated using 1ml of nutrient broth | ||
*The culture was aseptically transferred back into the broth containing tube | *The culture was aseptically transferred back into the broth containing tube | ||
| - | * | + | *Mix |
*From that tube, colonies were streaked on 6 plates and left to incubate over night at 30C | *From that tube, colonies were streaked on 6 plates and left to incubate over night at 30C | ||
Revision as of 12:41, 21 August 2013
Making nutrient agar plates and broth
- Making up broth and agar solution to grow P. putida in.
- For agar plates:
- 9.2g of nutrient agar medium
- 400ml of distilled water
- For broth:
- 3.2g of nutrient broth
- 400ml of distilled water
- Both were autoclaved for 45mins
- Agar was poured onto 16 plates
Plating out bacteria
- Freeze dried P. putida was rehydrated using 1ml of nutrient broth
- The culture was aseptically transferred back into the broth containing tube
- Mix
- From that tube, colonies were streaked on 6 plates and left to incubate over night at 30C
Growing bacteria in broth
- 100ml of autoclaved broth was added to 2 flasks each
- The broth tube, containing the bacteria, was divided between flasks
- Flask were left to incubate at 30C on a shaker overnight
