Team:Groningen/Labwork/22 August 2013

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<br>SS1 and SS2 do not show any colonies.
<br>SS1 and SS2 do not show any colonies.
<br><br>Negative and positive control show no colony and thousands of colonies, respectively.
<br><br>Negative and positive control show no colony and thousands of colonies, respectively.
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<h2>Sebas</h2>
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Transformation yesterday:
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<br> - negative control: 3 small colonies (somehow resistant to amp)
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<Br> - 100µl transformation mix: ~50 small colonies
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<Br> - On the extra concentrated plate (EC): ~300 small colonies + 11 big colonies
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<Br>
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<Br>Restreaked 6 of the big colonies on plate and grew them in 3ml LB/amp.
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Revision as of 09:05, 22 August 2013

Claudio

S1, S2, S5 and S6 show colonies.
SS1 and SS2 do not show any colonies.

Negative and positive control show no colony and thousands of colonies, respectively.

Sebas

Transformation yesterday:
- negative control: 3 small colonies (somehow resistant to amp)
- 100µl transformation mix: ~50 small colonies
- On the extra concentrated plate (EC): ~300 small colonies + 11 big colonies

Restreaked 6 of the big colonies on plate and grew them in 3ml LB/amp.