Team:BYU Provo/Notebook/SmallPhage/Winterexp/Period1/Explist

From 2013.igem.org

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<font size="4"> '''5.3 T7 Phage Selection Method Test''' </font>
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<font size="4"> '''3.16 Plan of Attack''' </font>
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In this experiment, we aimed to test whether or not we can select for smaller phages using concentrated top agar.
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After some background research we were able to come up with a plan of attack.
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 Phage Selection Method Test]]
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period1/Exp/PlanOfAttack|3.16 Plan of Attack]]
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<font size="4"> '''5.3 T7 Phage Amplification/Purification''' </font>
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<font size="4"> '''3.20 Phage Viability Test''' </font>
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In this experiment, we aimed to establish a basic protocol for amplifying and purifying phage.  
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We performed this experiment to familiarize ourselves with working with phage.  
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Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU Provo/5.3 Phage Amplification/Purification|5.3 T7 Phage Amplification/Purification]]
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Winterexp/Period1/Exp/3.20 Phage Viability Test|3.20 Phage Viability Test]]
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<font size="4"> '''5.5 Amplification From a Plaque Test''' </font>
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In this experiment, we aimed to test whether or not we can amplify a phage plaque using liquid culture
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.5_Amplification_from_a_plaque_test|5.5 Amplification From a Plaque Test]]
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<font size="4"> '''5.6 T7+ Liquid Culture Phage Concentration Test ''' </font>
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In this experiment, we aimed to test whether or not starting phage liquid culture with different concentrations of phage will affect the overall yield of phage progeny.
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.6_T7%2B_Liquid_Culture_Phage_Concentration_Test|5.6 T7+ Liquid Culture Phage Concentration Test ]]
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<font size="4"> '''5.9 T7 Selection Method Test #2''' </font>
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We performed this experiment to familiarize ourselves with x8 agar.
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Detailed procedure: [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/5.9_T7_selection_method_test|5.9 T7 Selection Method Test #2]]
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<font size="4"> '''5.9 T7+ Liquid Culture Phage Concentration Test #2''' </font>
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We repeated [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.6_T7%2B_Liquid_Culture_Phage_Concentration_Test|5.6 T7+ Liquid Culture Phage Concentration Test ]] to test for whether 1uL will give us even higher phage titer
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Detailed procedure: [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Exp/5.9 T7+ Liquid Culture Phage Concentration Test|5.9 T7+ Liquid Culture Phage Concentration Test #2]]
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Revision as of 00:45, 30 May 2013


Small Phage March - April Notebook: April 29 - May 12 Experiment Listing



Overview
March-April
May-June
July-August
September-October

3.16 Plan of Attack

After some background research we were able to come up with a plan of attack.

Detailed procedure: 3.16 Plan of Attack


3.20 Phage Viability Test

We performed this experiment to familiarize ourselves with working with phage.

Detailed procedure: 3.20 Phage Viability Test