"
18/07/13
From 2013.igem.org
(Difference between revisions)
AliceHaworth (Talk | contribs) (→Single digest) |
AliceHaworth (Talk | contribs) (→Single digest) |
||
| Line 18: | Line 18: | ||
*Incubate samples at 37C for 1h | *Incubate samples at 37C for 1h | ||
*2ul of orange G dye was added to each samples | *2ul of orange G dye was added to each samples | ||
| - | *Samples were then frozen overnight to run on gel | + | *Samples were then frozen overnight to run on gel on Monday |
Latest revision as of 14:16, 22 August 2013
Single digest
- Digesting RFP plasmid again using a single restriction enzyme- XbaI
- Digesting DNA samples 1-6
- 200ng of DNA is run
- Volumes for digest:
| Sample nr | 1 | 2 | 3 | 4 | 5 | 6 |
| DNA | 0.7 | 0.7 | 0.8 | 1.3 | 1.2 | 2.6 |
| Buffer | 2 | 2 | 2 | 2 | 2 | 2 |
| Water | 16.3 | 16.3 | 16.2 | 15.7 | 15.8 | 14.4 |
| XbaI | 1 | 1 | 1 | 1 | 1 | 1 |
- Buffer used was cutsmart
- Incubate samples at 37C for 1h
- 2ul of orange G dye was added to each samples
- Samples were then frozen overnight to run on gel on Monday
