Team:Groningen/Labwork/28 August 2013

Inne

Plates were taken out of the incubators at 10:20, they were incubated for 16 hours.
Plates were put back into incubators at 12:39.
A picture was taken at 15:00 plates were put back in incubator at 15:14
Inoculated strains form the 0.3 37C series in minimal medium, to see if this induces motility. Tubes were put in the 37C shaker for 5 hours, starting time 15:00
17:40 plates were taken out of incubator, pictures were taken. Plates were put back in their incubators at 18:01

Inoculated new (0.4% (that were spares)) platesvwith -80 stock of wt, delta cheY and delta cheY/delta des. In case minimal medium today fails. Inoculation was done in duplo with one with pasteur pipet and the other with toothpick.

21:45 The inoculations in minimal media, have turned turbid after 5 hours at 37C with shaking.
2,5 u/L of the inoculations were put on 0.4% and 0.3% plates. After drying the plates are put in incubators, one set is grown at 37C and one on 30C.
Plates were put in incubators at 22:05.

Claudio

S15 and S4 were digested with EcoRI+BamHI and PstI+BamHI respectively.
S15 and S4 were ligated into pSB1C3 (previously digested with EcoRI and PstI). The ligation reaction was incubated in the fridge overnight.