"
User contributions
From 2013.igem.org
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 02:50, 28 September 2013 (diff | hist) Decontaminating XL10 Gold (→Background) (top)
- 02:36, 28 September 2013 (diff | hist) Team:GeorgiaTech/Attributions (→Faculty)
- 02:17, 28 September 2013 (diff | hist) Decontaminating XL10 Gold
- 02:17, 28 September 2013 (diff | hist) N Decontaminating XL10 Gold (Created page with "{{:Team:GeorgiaTech/template}} ==Decontaminating XL10 Gold ==Purpose== To remake the XL10 Gold E. coli to remove the Ampicillin resistance that has contaminated the original. ==...")
- 02:14, 28 September 2013 (diff | hist) N Lambda Recombineering (Created page with "{{:Team:GeorgiaTech/template}} ==Lambda Recombineering== ==Purpose== To replace the ''dsbA'' from BL21(De3) strain of E. coli ==Background== Currently there is a collection o...") (top)
- 02:12, 28 September 2013 (diff | hist) Team:GeorgiaTech/Project (→Projects)
- 02:04, 28 September 2013 (diff | hist) Electroporation (top)
- 01:58, 28 September 2013 (diff | hist) Team:GeorgiaTech/Electrocompetent Cells Protocol (→Electrocompetent Cells Protocol) (top)
- 01:48, 28 September 2013 (diff | hist) N Electrocompetent Cells Protocol (Created page with "{{:Team:GeorgiaTech/template}} ==Electrocompetent Cells Protocol== # Grow media culture of the cells at 37 degrees # Inoculate flask with 500mL of SOB media with 1mL of the med...") (top)
- 01:42, 28 September 2013 (diff | hist) N Colony PCR Protocol (Created page with "{{:Team:GeorgiaTech/template}} ==Colony PCR Protocol== ===Materials=== *Prepare master mix: (Total volume '''50 µL''') **5X Rxn Buffer '''10 µL''' **Oligo 1 (5µM) '''7.5...") (top)
- 01:35, 28 September 2013 (diff | hist) N Chemically Competent Transformation (Created page with "{{:Team:GeorgiaTech/template}} ==Chemically Competent Transformation == ===Materials=== *Chemically Competent Cells -'''20 µL''' *PCR machine *10% BME ( beta mercaptoethanol ) ...") (top)
- 01:32, 28 September 2013 (diff | hist) N Gel Electrophoresis Protocol (Created page with " {{:Team:GeorgiaTech/template}} == Gel Electrophoresis Protocol == ==Procedure== *Get a agarose gel from the refrigerator and place in apparatus, making sure it is comple...") (top)
- 01:17, 28 September 2013 (diff | hist) Team:GeorgiaTech/Electrocompetent Cells Protocol
- 01:16, 28 September 2013 (diff | hist) Chemically Competent Cells Protocol (top)
- 01:15, 28 September 2013 (diff | hist) COLONPCR Protocol (→COLONY PCR Protocol for PCR machines) (top)
- 01:14, 28 September 2013 (diff | hist) COLONPCR Protocol (→COLONY PCR Protocol for PCR machines)
- 01:13, 28 September 2013 (diff | hist) Team:GeorgiaTech/Colony PCR Protocol (top)
- 01:13, 28 September 2013 (diff | hist) Team:GeorgiaTech/Colony PCR Protocol
- 01:10, 28 September 2013 (diff | hist) Agar Plates and Media Protocol (top)
- 00:56, 28 September 2013 (diff | hist) N Chemically Competent Cells Protocol (Created page with "{{:Team:GeorgiaTech/template}} Preparation of Competent Cells 1. Pick a colony from plate using a toothpick and drop toothpick into 5mL of SOB media. Let this starter culture i...")
- 00:49, 28 September 2013 (diff | hist) N Agar Plates and Media Protocol (Created page with "{{:Team:GeorgiaTech/template}} *Add (35 g/L of LB Agar) or (25 g/L LB broth and 15 g/L Agar) to a flask with distilled water *Dissolve and autoclave for 1 ...")
- 00:41, 28 September 2013 (diff | hist) Team:GeorgiaTech/Chemically Competent Transformation (→Materials) (top)
- 00:41, 28 September 2013 (diff | hist) Team:GeorgiaTech/Chemically Competent Transformation (→Estimated Time: 1 hr 11 mins)
- 00:39, 28 September 2013 (diff | hist) Team:GeorgiaTech/Chemically Competent Transformation (→Estimated Time: 1 hr 11 mins)
- 20:42, 27 September 2013 (diff | hist) N File:IMAG0033.jpg (top)
- 20:38, 27 September 2013 (diff | hist) N Electroporation (Created page with "In order to transform electro competent cells, a specific protocol must be followed: # Chill cuvettes for 1 hour at 4 degrees Celsius prior to performing this transformation. # ...")
- 20:37, 27 September 2013 (diff | hist) Team:GeorgiaTech/Lambda Recombineering (→Design)
- 20:35, 27 September 2013 (diff | hist) N File:BK-igem13-Recombineering (1).pdf (top)
- 20:34, 27 September 2013 (diff | hist) N Team:GeorgiaTech/Lambda Recombineering (Created page with "==Purpose== To replace the ''dsbA'' from BL21(De3) strain of E. coli ==Background== ==Starting Materials== *Vector containing Lambda Red Recombinase (Temperature Sensitive 30...")
- 20:30, 27 September 2013 (diff | hist) Team:GeorgiaTech/Chemically Competent Transformation (→Procedure)
- 20:29, 27 September 2013 (diff | hist) Team:GeorgiaTech/Chemically Competent Transformation
- 20:27, 27 September 2013 (diff | hist) N Team:GeorgiaTech/Chemically Competent Transformation (Created page with "===Materials=== *Chemically Competent Cells -'''20 µL''' *PCR machine *10% BME ( beta mercaptoethanol ) - '''0.88 µL''' *SOC media - '''222 µL''' *Pipette tips with cut ends (...")
- 20:25, 27 September 2013 (diff | hist) N Team:GeorgiaTech/Gel Electrophoresis Protocol (Created page with "==Procedure== *Get a agarose gel from the refrigerator and place in apparatus, making sure it is completely covered in TAE buffer. *Calculate the amount of purified plas...") (top)
- 20:22, 27 September 2013 (diff | hist) Team:GeorgiaTech/Electrocompetent Cells Protocol
- 20:21, 27 September 2013 (diff | hist) Team:GeorgiaTech/Electrocompetent Cells Protocol
- 20:19, 27 September 2013 (diff | hist) N Team:GeorgiaTech/Electrocompetent Cells Protocol (Created page with "# Grow media culture of the cells at 37 degrees # Inoculate flask with 500mL of SOB media with 1mL of the media culture # Place flask in the 37 degree Celsius shaker. # Check th...")
- 20:14, 27 September 2013 (diff | hist) Team:GeorgiaTech/Agar Plates and Media Protocol (top)
- 20:08, 27 September 2013 (diff | hist) N Team:GeorgiaTech/Chemically Competent Cells Protocol (Created page with "Preparation of Competent Cells 1. Pick a colony from plate using a toothpick and drop toothpick into 5mL of SOB media. Let this starter culture incubate overnight at 37 celsius ...") (top)
- 20:06, 27 September 2013 (diff | hist) N COLONPCR Protocol (Created page with "===COLONY PCR Protocol for PCR machines=== #98ºC for '''2 min''' #98ºC for '''15 sec''' #60ºC for '''15 sec''' #72ºC for '''2 min''' #repeat steps 2-4 '''31 times''' #72ºC f...")
- 20:05, 27 September 2013 (diff | hist) N Team:GeorgiaTech/Colony PCR Protocol (Created page with "===Materials=== *Prepare master mix: (Total volume '''50 µL''') **5X Rxn Buffer '''10 µL''' **Oligo 1 (5µM) '''7.5 µL''' **Oligo 2 (5µM) '''7.5 µL''' **dNTP mix ...")
- 19:54, 27 September 2013 (diff | hist) N Team:GeorgiaTech/Agar Plates and Media Protocol (Created page with "*Add (35 g/L of LB Agar) or (25 g/L LB broth and 15 g/L Agar) to a flask with distilled water *Dissolve and autoclave for 30 mins (Basement lab [[Autoclave ...")
- 14:12, 27 September 2013 (diff | hist) Team:GeorgiaTech/Decontaminating XL 10 Gold (→Background) (top)
- 14:09, 27 September 2013 (diff | hist) Team:GeorgiaTech/Decontaminating XL 10 Gold (→Purpose)
- 13:57, 27 September 2013 (diff | hist) N File:20130629 175104.jpg (top)
- 13:55, 27 September 2013 (diff | hist) Team:GeorgiaTech/Decontaminating XL 10 Gold (→Starting Materials)
- 13:55, 27 September 2013 (diff | hist) Team:GeorgiaTech/Decontaminating XL 10 Gold (→Background)
- 13:53, 27 September 2013 (diff | hist) N File:20130629 175052.jpg (top)
- 13:52, 27 September 2013 (diff | hist) Team:GeorgiaTech/Decontaminating XL 10 Gold (→June 29th, 2013)
- 13:51, 27 September 2013 (diff | hist) N File:Plate1.jpg (top)
- 13:47, 27 September 2013 (diff | hist) N Team:GeorgiaTech/Decontaminating XL 10 Gold (Created page with "==Purpose== To remake the XL10 Gold E. coli to remove the Ampicillin resistance that has infected the original. ==Background== We have noticed that our XL10 Gold Competent cells...")
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
