From 2013.igem.org
Aug 26
Matsumoto and Ashida
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | -
|
| 2 | 8/24 tetR aptamer 12_P | EcoRI&SpeI
|
| 4 | 8/24 tetR aptamer 12_1R | EcoRI&SpeI
|
| 6 | 8/24 tetR aptamer 12_M | EcoRI&SpeI
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230
|
| tetR aptamer 12_P(EcoRI&SpeI) | 2.3 | 0.38 | 0.13
|
| tetR aptamer 12_1R(EcoRI&SpeI) | 12.0 | 0.63 | 1.19
|
| tetR aptamer 12_M(EcoRI&SpeI) | 2.0 | 6.43 | 0.03
|
Ligation
Ashida
| state | Vector | Inserter | Ligation High ver.2
|
| experiment | 8/21 DT | 5.0 | 8/26 tetR aptamer 12_1R | 2.0 | 3.5
|
| experiment | 8/21 DT | 5.0 | 8/22 pT181 atteniator | 5.0 | 5.0
|
| experiment | 8/21 DT | 5.0 | 8/22 pT181 antisense | 3.0 | 4.0
|
| experiment | 8/21 DT | 5.0 | 8/22 Spinach | 3.0 | 4.0
|
| experiment | 8/22 Plac | 4.0 | 8/22 pT181 attenuator | 5.0 | 4.5
|
| experiment | 8/22 J23100 | 4.0 | 8/22 pT181 attenuator | 5.0 | 4.5
|
| experiment | 8/22 Plac | 4.0 | 8/22 pT181 antisense | 8.0 | 6.0
|
| experiment | 8/22 Plac | 4.0 | 8/22 pT181 antisense | 8.0 | 6.0
|
| experiment | 8/22 Pcon | 4.0 | 8/22 pT181 antisense | 8.0 | 6.0
|
Transformation
No name
| Name | Sample | Competent Cells | Total | Plate
|
| 8/26 tetR aptamer 12_1R-DT | 1µL | 10µL | 11µL | CP
|
| 8/26 pT181 attenuator-DT | 1µL | 10µL | 11µL | CP
|
| 8/26 pT181 antisense-DT | 1µL | 10µL | 11µL | CP
|
| 8/26 Spinach-DT | 1µL | 10µL | 11µL | CP
|
| 8/26 Plac-pT181 attenuator | 1µL | 10µL | 11µL | CP
|
| 8/26 Pcon-pT181 attenuator | 1µL | 10µL | 11µL | Amp
|
| 8/26 Plac-pT181 antisense | 1µL | 10µL | 11µL | CP
|
| 8/26 Pcon-pT181 antisense | 1µL | 10µL | 11µL | Amp
|
| Pλ-RBS-luxI-DT | 1µL | 10µL | 11µ | Amp
|
"
