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From 2013.igem.org

Contents 1 DNA isolation from PCR products 2 Nanodropping the samples for concentration 3 Sending isolated DNA to be sequenced by PNACL 4 Preparing overnight broths

DNA isolation from PCR products

• Isolating DNA using Omega nucleic acid purification kit and following its protocol.
• Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
• Selection was made by looking at gel pictures and selecting samples with visible bands.

Nanodropping the samples for concentration

Sample nr	Volume ul	Concentration ng/ul	260/280	260/230
X3	41	11.3	1.87	1.29
X5	47	12.2	1.78	1.22
X6	34	12.9	1.79	1.22
X7	45	8	1.71	0.96
X8	43	10.5	1.88	1.2
X10	44	9	1.88	1.2
X12	45	12.8	1.70	1.40
F1	44.5	11.2	1.69	1.22
F2	44	9.1	1.88	1.20
F3	45.2	10.6	1.77	0.99
F4	43.7	9.7	1.82	1.10
F5	45.2	10.6	1.67	1.19
F6	45.4	9.6	1.82	0.85
F7	46	11	1.76	1.14
F8	46	11.7	1.73	1.24
F9	42.4	10.5	1.83	1.32
F10	44.3	11	1.93	1.39
F11	45.5	10.3	1.82	1.18
F12	45.5	14.9	1.83	1.32
B1	45	6.9	1.56	0.86
B2	46	10.5	1.64	1.17
B3	46	10	1.78	1.41
B4	47	11.5	1.70	1.35
B5	47	10.4	1.84	1.25
B6	47	8.6	1.96	1.33
B7	47	9.7	1.74	1.01
B8	46	10.7	1.22
B9	45	12.4	1.58	1.05
B10	46	11.1	1.73	1.32
B11	46	10.2	1.87	1.56
B12	46	12.1	1.75	1.38

Sending isolated DNA to be sequenced by PNACL

• Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.

Preparing overnight broths

• Preparing broths from individual colonie plates from previous day
• Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.