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19/09/13
From 2013.igem.org
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Contents |
DNA isolation from PCR products
- Isolating DNA using Omega nucleic acid purification kit and following its protocol.
- Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
- Selection was made by looking at gel pictures and selecting samples with visible bands.
Nanodropping the samples for concentration
| Sample nr | Volume ul | Concentration ng/ul | 260/280 | 260/230 |
| X3 | 41 | 11.3 | 1.87 | 1.29 |
| X5 | 47 | 12.2 | 1.78 | 1.22 |
| X6 | 34 | 12.9 | 1.79 | 1.22 |
| X7 | 45 | 8 | 1.71 | 0.96 |
| X8 | 43 | 10.5 | 1.88 | 1.2 |
| X10 | 44 | 9 | 1.88 | 1.2 |
| X12 | 45 | 12.8 | 1.70 | 1.40 |
| F1 | 44.5 | 11.2 | 1.69 | 1.22 |
| F2 | 44 | 9.1 | 1.88 | 1.20 |
| F3 | 45.2 | 10.6 | 1.77 | 0.99 |
| F4 | 43.7 | 9.7 | 1.82 | 1.10 |
| F5 | 45.2 | 10.6 | 1.67 | 1.19 |
| F6 | 45.4 | 9.6 | 1.82 | 0.85 |
| F7 | 46 | 11 | 1.76 | 1.14 |
| F8 | 46 | 11.7 | 1.73 | 1.24 |
| F9 | 42.4 | 10.5 | 1.83 | 1.32 |
| F10 | 44.3 | 11 | 1.93 | 1.39 |
| F11 | 45.5 | 10.3 | 1.82 | 1.18 |
| F12 | 45.5 | 14.9 | 1.83 | 1.32 |
| B1 | 45 | 6.9 | 1.56 | 0.86 |
| B2 | 46 | 10.5 | 1.64 | 1.17 |
| B3 | 46 | 10 | 1.78 | 1.41 |
| B4 | 47 | 11.5 | 1.70 | 1.35 |
| B5 | 47 | 10.4 | 1.84 | 1.25 |
| B6 | 47 | 8.6 | 1.96 | 1.33 |
| B7 | 47 | 9.7 | 1.74 | 1.01 |
| B8 | 46 | 10.7 | 1.86 | 1.22 |
| B9 | 45 | 12.4 | 1.58 | 1.05 |
| B10 | 46 | 11.1 | 1.73 | 1.32 |
| B11 | 46 | 10.2 | 1.87 | 1.56 |
| B12 | 46 | 12.1 | 1.75 | 1.38 |
Sending isolated DNA to be sequenced by PNACL
- Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.
Preparing overnight broths
- Preparing broths from individual colonie plates from previous day
- Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.
