Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.31 EM Pictures

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Phage Purification July - August Notebook: Experiments

7.31 EM Pictures

I) Purpose

To see if we have mutated, pure phage from the CsCl gradient and mutagenisis

II) Expected Outcome

Pictures of pure, intact phage, potentially mutated phage.

III) Reagants Used

Prepared Phage

IV) Actual Procedure

Gave prepared phage to TEM operator. We had four sample types:

Phage from the 'petite' band in the CsCl gradient

Phage from the 'regular/wildtype' band in the CsCl gradient

Phage from the 'mutant' band in the CsCl gradient

Phage that had 2x mutagen

V) Results

Pictures of Phage

Petite Phage

.

Wild Type Phage

Mutated Phage

Mutated Phage - 2x Mutagen