Team:UNIK Copenhagen/Signe Notebook
From 2013.igem.org
Notebook
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Week 1
8th-14th of July
Culturing of magnetotactic bacteria (MTBs)
Making base media for growing magnetotactic bacteria. This media was also used to make agar plates.
MS-1 inoculated in liquid base medium. Falcon tubes were flushed with the nitrogen before use and incubated at 28 degrees.
Plates were kept in a closed container with an Oxoid AnaeroGen pad.
Assembly of constructs
Glycerol stock of E. coli with pBBR1MCS-2 was thawed to grow on these plates.
Primers for MamC (MS-1 strain) was ordered.
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