siRNA screening (Failed)14th: We extracted plasmids and cultured the 293t cells.15th: We transfected plasmids into 293t cells.16th: We collected cells and preserved it in Trizol.17th: We extracted RNA, then did RT-PCR with the RNA.18th: We did qPCR with the cDNA we got on 17th April.21th: We reexamined the concentration of RNA, and redid qPCR.
siRNA screening (Failed)27th: We cultured the 293t cells in a 12-well plate.28th: We transfected 293t cells.29th: We collected cells and extracted RNA from it.30th: We did RT-qPCR with the RNA we extracted on 29th April.1st: We did qPCR with the cDNA we got on 30th April.
siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success)8th: We extracted plasmids of 3 kinds of siRNA.9th: We cultured 293t cells in eight D10 dishes and a 12-well plate.10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively.11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate.12th: We extracted RNA from cells and exosomes.13th: We did RT-PCR and qPCR.
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