Team:Chiba/Assay/oxidation

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iGEM-2013 Chiba

Experiment for measurement of oxidative state

1. Dissolved methylene blue into distilled water and made 10 mM methylene blue solution.
2. Sterilized methylene blue solution using a filter.
3. Took 8mL of diluted methylene blue solution as shown in Fig.X and mixed with 125% LB culture 32mL.
4. precultured BL21and shuffle for 12 hours being cultured on methylene blue plates.