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From 2013.igem.org

Overview

We are utilizing the pOSIP plasmid to chromosomally integrate transformed genes into NEB 10 Beta cells and then remove antibiotic resistance genes to prevent horizontal gene transfer of LLO or any antibiotic resistance genes. We are also developing a pH-based gene switch to create a secondary safety mechanism so that LLO is only translated under acidic conditions and translation is repressed by neutral and basic conditions.

pOSIP

pOSIP is a system used to directly integrate genes into the genome of an organism. The ASU iGEM team plans to use this vector to deliver GFP and LLO directly into the genome to create a modular platform for cancer vaccine delivery, which can be altered simply by switching out a plasmid containing the cancer antigen of interest

LLO pH Switch