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From 2013.igem.org

Use of Ubiquitin as a Mechanism for MHC Class I Presentation

Ubiquitin is a small 8.5 kDa regulatory protein. It is post-translationally added to substrate proteins, signaling protein lysis, protein localization, or activation/inhibition of protein function. In the future, we plan to create a fusion protein of FluM1 and MelanA pathogenic antigens with ubiquitin, signaling endocytosis and viral budding of the our antigen, leading to cytotoxic T cell response.

Dendritic Cell Targeting via aDEC-205 ScFv

The ant-DEC205 single chain variable fragment is an antibody fragment that binds to the DEC205 surface protein on dendritic cells. We plan to express this scFv on the surface of our vaccine chassis using a surface anchor protein, such as ice nucleation protein, to increase its affinity for dendritic cell targeting and uptake.