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From 2013.igem.org

Main purpose today

Make M9 Medium for the biolector toxicity experiment

Who was in the lab

Helen, Gosia, Ariadni

Procedure

According to open wetware protocol for Biolector Experiment

1L of Media:

• 10 mL Casamino Acid ( ready solution from Andreas)
• 2 mM MgSO4 (from 2M liquid stock)
• 0.1 mM CaCl2 (liquid solution with 15% gly)
• 200 mL M9 salts
• Fill to 1 L with sterilized water

1 L stock of M9 salts:

• 64 g Na2HPO4*7H2O = 31.2 g Na2HPO4
• 15 g KH2PO4
• 25 g NaCl
• 5 g NH4Cl