Team:BYU Provo/Notebook/SmallPhage/Springexp/Period4/Dailylog

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Small Phage May - June Notebook: June 17 - June 30 Daily Log

Small Phage

6/17/13

- Plated 5 controls and 50 selection plates (500ug at -2) as part of selection 1 in 6.12 Mutagen Concentration Test - Second Protocol

6/18/13

- Started approximately 50mL of BL21 liquid culture overnight.

6/19/13

- We performed Large Plaque Confirmation 1 as part of 6.12 Mutagen Concentration Test - Second Protocol

6/23/13

- Started 40mL of BL21 liquid culture overnight.

6/24/13

- Made more x8 top agar. - Because the large plaque confirmation 1 had contamination from overnight (the LB was contaminated the next day), we redid the confirmation procedure: Large Plaque Confirmation 2 in 6.12 Mutagen Concentration Test - Second Protocol.

6/26/13

- Made about 50ml of BL21 liquid culture overnight

6/27/13

- Started the mutagenesis procedure for 6.27 Mutagen Concentration Test - Third Protocol

6/28/13

- Performed first round of titer for 6.27 Mutagen Concentration Test - Third Protocol. This includes 0, -2, -4, -6, and -8 for 0ug, 100ug, 200ug, and 500ug mutagen concentration.