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Sensing

OD measurements
I let the bacteria with the three promoters hya, cad and constitutive grow in media with different pHs. Each 45-70 minutes I measured the OD in order to see if the absence of GFP expression in the plate reader was due to non- induction of the promoter or the simple fact that the bacteria were dying at ceratin pH values. Indeed this experiments showed, that the bacteria in the acidic milieu died very quickly which could explaind why the promoter was never induced.