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Cell Surface Display

Gibson Transformation
-Transformation of HB5α competent cells with INP_Strep-Dead gibson product previously obtained.
-Overnight incubation of the transformed cells in chloramphenicol paltes at 37°C.
-Overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.

Nanoparticles

Making Nanoparticles, 1st try
- Purification of the digested GPs.
- Measurement of the GPs+dye with Dynamic Light Scattering (DLS). Results: good, we get some 10nm particles and a few 100nm. We hope they are nanoparticles and not artifacts.
- Started a new stock of GPs; made the gelatin solution, cross-linked with glutaraldehyde and stirred overnight.