Results

Over the summer Team Magneto worked hard and some of the preset goals were successfully accomplished. Here we show and explain you our main results.

Quick Overview

• MamC-eGFP: the functionality of the eGFP part of the fusion was proven, regarding the MamC part only the expected size of the protein could be shown

• MamC alone couldn’t be further characterized

• eGFP: the functionality and autenticity of the protein could be shown

• eFbFP: the submitted BioBrick couldn’t be successfully characterized

MamC-eGFP

MamC (BBa_K1094001) from Magnetospirillum magnetotacticum (MS-1) was tagged with enhanced green fluorescent protein (eGFP, BBa_K1094400). Between the parts a glycine linker (10 glycine residues) is added. The gene product can be used to detect localization of MamC in MS-1. The two parts were cloned together using classical cloning into pJAM1786 in E. coli. Colony PCR and gel electrophoresis was performed to ensure the insert was in the vector (Fig.1).