Team:TU Darmstadt/materials/Media

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Lab book | Materials | Protocols

Media

1.7-DYT (recipe for 500 mL)

  • 2.5 g NaCl
  • 8.0 g tryptone
  • 5.0 g yeast extract
  • add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

LB (recipe for 1000 mL)

  • 10 g tryptone
  • 5 g yeast extract
  • 10 g NaCl
  • add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
  • for plates: add 15g agar

SOB (recipe for 1000 mL)

  • 0.186 g KCl
  • 2.4 g MgSO4
  • 0.584 g NaCl
  • 20 g tryptone
  • 5 g yeast extract
  • add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

SOC (recipe for 1000 mL)

  • 980 mL SOB
  • 20 mL 1M Glucose

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