Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period4/Dailylog

Small Phage

March-April

May-June

July-August

September-October

• Plated for mutants as part of 10.8 Characterization of Mutant Phage.

10/18/13

JL, LP

• Performed amplification and purification of insert as part of 10.18 Cloning T7 Capsid Protein into iGEM Registry.

10/19/13

JL, LP

• Ran a low melt gel for 10.18 Cloning T7 Capsid Protein into iGEM Registry.

10/20/13

JL

• Performed the repeat PCR and gel step in 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/21/13

JL, LP

• Did restriction digest for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/22/13

JL

• Low-melt gel for purification of sticky-ended insert/vector for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/23/13

JL, LP

• Did ligation and transformation for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/24/13

JL, LP

• Did Colony PCR and Overnights for Vector Purification for 10.18 Cloning T7 Capsid Protein into iGEM Registry

10/25/13

JL, LP

• Did plasmid cleanup for 10.18 Cloning T7 Capsid Protein into iGEM Registry and sent the plasmid into iGEM!

10/27/13

JL

• Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for 10.8 Characterization of Mutant Phage.

10/28/13

JL

• Propagated WT, S4, S10, S21, S22, and L8 for 10/27/13

JL, LP

• Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for 10.8 Characterization of Mutant Phage.

• Mapped out mutation for S4 capsid protein.

• Finished the wiki.