Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Exp/10.16CsClGradient

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Phage Purification September - October Notebook: Experiments

Phage Purification March-April May-June July-August September-October

10.16 T7 EM I) Purpose Identify and measure capsid sizes of mutated phage. II) Expected Outcome Small and large mutated phage. III) Reagants Used T7 mutant phage TEM kit IV) Actual Procedure Place 10 microL of sample onto EM grid. Let sit for 1 min. Dry by touching a dry piece of material to the side of the grid. (provided in EM kit). Place 10 microL EM dye onto grid. Wait 90 seconds before drying. Place in EM grid carrier. Deliver to the TEM operator for imaging. V) Results Found 3 smaller mutants and 1 large mutant. We will take more pictures to measure more capsid sizes. T7 First Gradient T7 Second Gradient