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Nucleotide sequence in RFC 10: (underlined part encodes the protein)
GTACACAATGCGTCGT ... TTCGAAAAATAA
ORF from nucleotide position 8 to 1705 (excluding stop-codon) |
Amino acid sequence: (RFC25 scars in shown in bold, other sequence features underlined; both given below)
| 1 | MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE | | 101 | FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW | | 201 | FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL | | 301 | DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT | | 401 | ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE | | 501 | FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK* |
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Sequence features: (with their position in the amino acid sequence, see the list of supported features) | RFC25 scar (shown in bold): | 556 to 557 | | Strep-tag II: | 559 to 566 |
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Amino acid composition:| Ala (A) | 33 (5.8%) | | Arg (R) | 25 (4.4%) | | Asn (N) | 27 (4.8%) | | Asp (D) | 34 (6.0%) |
| | Cys (C) | 4 (0.7%) | | Gln (Q) | 24 (4.2%) | | Glu (E) | 48 (8.5%) | | Gly (G) | 29 (5.1%) |
| | His (H) | 18 (3.2%) | | Ile (I) | 39 (6.9%) | | Leu (L) | 64 (11.3%) | | Lys (K) | 36 (6.4%) |
| | Met (M) | 16 (2.8%) | | Phe (F) | 28 (4.9%) | | Pro (P) | 17 (3.0%) | | Ser (S) | 33 (5.8%) |
| | Thr (T) | 26 (4.6%) | | Trp (W) | 14 (2.5%) | | Tyr (Y) | 27 (4.8%) | | Val (V) | 24 (4.2%) |
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Amino acid counting | Total number: | 566 | | Positively charged (Arg+Lys): | 61 (10.8%) | | Negatively charged (Asp+Glu): | 82 (14.5%) | | Aromatic (Phe+His+Try+Tyr): | 87 (15.4%) |
| Biochemical parameters | Atomic composition: | C3002H4586N778O868S20 | | Molecular mass [Da]: | 66105.3 | | Theoretical pI: | 5.38 | | Extinction coefficient at 280 nm [M-1 cm-1]: | 117230 / 117480 (all Cys red/ox) |
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Codon usage | Organism: | E. coli | B. subtilis | S. cerevisiae | A. thaliana | P. patens | Mammals | | Codon quality (CAI): | good (0.71) | good (0.75) | good (0.69) | good (0.78) | codon usage physco | good (0.68) |
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