• Team The HokkaidoU_Japan is formed by 16 students and 3 instructors
• Project We tried to further develop E. coli-based protein injection system which we started working on during iGEM 2010
• Parts Ready-to-inject backbone and Bsa I cloning system
• Protocol General protocols, Primers and methods of infection assay
• Notebook The record of our many failures and a bit of achievement
• Safety Addressing safety concerns
• Human Practice Science art gallery "BIO ART"

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Abstract

We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.

We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.

In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.

BioArt

We held the new type science art gallery "Bio Art" at the center of Sapporo city.

This gallery is not only for our team.You can also enjoy Bio Art on this Web page. You can watch more detail in Human Practice page.

Contents 1 Acknowledgements 1.1 Laboratory and Personnel 1.2 Academic Institutions 1.3 Companies

Acknowledgements

Laboratory and Personnel

• Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University

Ken-ichi Yamazaki and Members of Yamazaki Lab

• Molecular Biology, Institute for Genetic Medicine, Hokkaido University

Hisatoshi Shida

• Nikon Imaging Center

Takeharu Nagai, Kenta Saito, Masahiro Nakano, Kentarou Kobayashi, Hiroshi Nishino

• Laboratory of Molecular and Cellular Biophysics, Hokkaido University

Tomomi Nemoto, Terumasa Hibi, Ryosuke Kawakami

• Muroran Marine Station Field Science Center for Northern Biosphere, Hokkaido University

Taizo Motomura

• Laboratory of X-ray structural biology, Department of Advanced Transdisciplinary Science, faculty of Advanced Life Science

Isao Tanaka

• Research Institute for Electronic Science, Hokkaido University

Academic Institutions

• Salmonella Genetic Stock Center/University of Calgary
• Graduate School of Environmental Earth Science
• Department of biological science/School of Sciense
• Institute for Genetic Medicine

Companies

• Primary Cell Co., Ltd.
• ECONiXE Co., Ltd.
• Hokuto Kohki Engineering Co,. Ltd.
• COSMO BIO Co., Ltd.
• AMINO UP CHEMICAL Co., Ltd.
• Mendel Workshop Co., Ltd.

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