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08/08/13
From 2013.igem.org
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Making chloroamphenicol/IPTG plates
- 400ml of agar
- Boil for 3min x2
- Boil for 2min x2
- Cool to 40C
- Add 800ul of chloroamphenicol at 25um/ml
- Add 400ul IPTG at 0.15um/ml
- Makes 20 petri dishes
Streaked out samples of the limonene/pSB1C3 ligated plasmid
- Streaked samples 5.1, 10.1, 10.2 and 10.3 onto the chloroamphenicol/IPTG plates
- IPTG was used to induce the lac operon, and therefore induce limonene synthesis
- Plates were labelled using special symbols to ensure double-blinded test
