Team:Goettingen/NoteBook w1
From 2013.igem.org
June 6th, Thursday
Pick the colonies of part1-7
Media preparation1000ml LB+Amp Solid medium => about 50 Plates(with black code)
TransformationEntry Numer | Location on the kit | |||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
BBa_B0034 | P5 2 M | Pick the colonies of Part1-7
Code Name | Description |
---|---|
iGEM_36 | DarR operator sequence + biobrick prefix |
iGEM_37 | DarR operator sequence + biobrick sufix |
Transformation
Biobrick entry number | Mark |
---|---|
BBa_J23117 | part 1 |
BBa_J23116 | part 2 |
BBa_J23110 | part 3 |
BBa_J23118 | part 4 |
BBa_J61101 | part 5 |
BBa_BE0240-Cm | part 6 -- Cm |
BBa_B0015 | part 7 -- Cm |
BBa_B0034 | part 8 |
Resuspended but not transformed | |
Entry number | location on kit |
BBa_E0204-Amp | P5 12 M |
BBa_QO3121 | P5 20 N |
June 4th, Tuesday
Find the correct DNA sequence of DarR and primer design
Code Name | Description |
---|---|
iGEM_32 | Forward primer for DarR ORF amplification, with biobrick prefix |
iGEM_33 | Reverse primer for DarR ORF amplification, with biobrick sufix |
iGEM_34 | Forward primer for DarR ORF PCR amplification sequencing. |
iGEM_35 | Reverse primer for DarR ORF PCR amplification sequencing. |