Team:Groningen/Labwork/17 August 2013
From 2013.igem.org
Inne
For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.The hy-spank GFP e.coli plates grew colonies, 4 single colonies will be picked and inoculated.
Performed a restriction digest of ms2 samples 1 till 3.
0.8% gel was loaded as following.
slot 1 | slot 2 | slot 3 | slot 4 | slot 5 | slot 6 | slot 7 | slot 8 | slot 9 | slot 10 |
1kb DNA ruler | ms2 1 | ms2 2 | ms2 3 | uncut plasmid ms2 1 | 1kb DNA ruler | ms2 1 | ms2 2 | ms2 3 | uncut plasmid ms2 1 |