Team:Paris Bettencourt/Notebook/TB-ception/Thursday 29th August.html

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TB-ception

Thursday 29^th August

We did a phagocytosis assay for our macrophages. First we passaged our cells and we determined their number (colored them with trypan blue and counted them): 2.68* 10⁶, 3.72* 10⁶ and 1.32* 10⁶. To the first one we added 40, to the second 30 and to the last one 90 µl of NEB + pSB1A3 cell suspension. After 1 h we made this awesom picture:

Cloning from yesterday didn't work, so we repeated everything with longer digestion time for the backbone.