16/09/13

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Digesting pSB1C3 backbone

Digesting for ligation with the already digested Tod operon PCR products
Following the iGEM official protocol
Using EcoRI-HF and PstI-HF
Exluding the DpnI from the master mix and adjusting the volume of water accordingly
Protocol found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones]

Running the backbone and RFP on gel

on 1% gel to check if DNA was extracted on Friday

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