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From 2013.igem.org

Digesting pSB1C3 backbone

• Digesting for ligation with the already digested Tod operon PCR products
• Following the iGEM official protocol
• Using EcoRI-HF and PstI-HF
• Exluding the DpnI from the master mix and adjusting the volume of water accordingly
• Protocol can be found at: [http://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones]

Digestion of the TOD genes PCR products

The PCR products of TODX, TODF and TOBG were digested with the enzymes EcoRI-HF and PstI-HF. Protocol

Running the backbone and RFP on gel

• on 1% gel to check if the gel extraction worked.