Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.10PhagePreparation

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Phage Purification May - June Notebook: Experiments

6.10 Bacteria Cultures

I) Purpose

Grow bacteria for later use in preparation of phage.

II) Expected Outcome

Viable liquid cultures of both W3110 and BL21

III) Reagants Used

W3110 and BL21 freezer stock

LB

IV) Actual Procedure

Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37^◦ C for 1 hour.

Infect 4 mL of LB with 1 mL of liquid culture prepared in previous step.

Let both liquid cultures sit overnight at 37^◦ C.

V) Results

We were able to grow viable cultures of W3110 and BL21.