19/09/13

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Contents

DNA isolation from PCR products

  • Isolating DNA using Omega nucleic acid purification kit and following its protocol.
  • Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
  • Selection was made by looking at gel pictures and selecting samples with visible bands.

Nanodropping the samples for concentration

Sample nrVolume ulConcentration ng/ul260/280260/230

Sending isolated DNA to be sequenced by PNACL

  • Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.

Preparing overnight broths

  • Preparing broths from individual colonie plates from previous day
  • Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.
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