Template:Kyoto/Notebook/Aug 10

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Contents

Aug 10

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+amp)
8/9 BBa-J23100-1LB(+amp)
8/9 BBa-J23100-2LB(+amp)
8/9 BBa-J44000-1LB(+amp)
8/9 BBa-J44000-2LB(+amp)

Waking Up Parts

Nakamoto

Sample
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3
BBa-B0034(RBS)pSB1A2
BBa-I732020(RBS-lacZα-DTt)pSB1C3
BBa-P0412(RBS-lacI-DT)pSB1C3
BBa-R0011(Plac)pSB1C3

LB Medium Plate

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4µ NaOHaq50µL
Agar Pouder2g 

Transformation

Gel Making