Team:Groningen/Labwork/25 September 2013

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Mirjam

Two colonies are chosen and inoculated in LB medium with Cm to higher the amount of the Pdes-cheY construct.
Inoculations of ΔDesΔCheY and WT bacillus strains in minimal medium.
Did a gel purification of CheC up and CheC down. Did restriction analysis of CheC up, CheC down and Spec. Ligation of CheC. Transformation of CheC in the ΔDesΔCheY strain.