Team:Chiba/Project/store

Storage

1.Introduction

    Fe(Ⅱ) in E.coli causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to E.coli. As a result, giving iron to E.coli excessively leads to the death of E.coli.
    Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O₂→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe₂O₃(H₂O) has paramagnetism.
    These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.

2.1.Plasmid

パーツの事書く




Part link

2.2.Expression check

SDS pageについて書く

3.Results & Discussion

鉄を貯めるということは・・・・・

3.1.鉄耐性評価

Assay実験方法別ページ
実験結果書いてく

3.2.鉄取り込み量評価

Assay実験方法別ページ
実験結果書いてく

3.3.磁性評価

Assay実験方法別ページ

4.Conclusion

それは・・