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Contents 1 September 1.1 9/16/13 1.2 9/18/13 1.3 9/27/13

September

9/16/13

Today Jade and LJ helped us spot test all of the fractions above the band from our last CsCl gradient. These fractions were numbered 12-19, but 15 was skipped. Each fraction was spot tested at a titer of 10^0, 10^-2, and 10^-4 and incubated agar side down at 37C overnight.

9/18/13

We looked at the results from the spot test today. We found that: All fractions had phage at 10^0 Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques. Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4.

We also measured the capsids and tails of the TEM images.

9/27/13

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