Team:Chiba/Project/store

Storage

1.Introduction

    Fe(II) in E.coli causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to E.coli. As a result, giving iron to E.coli excessively leads to the death of E.coli.
    Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(II)＋O₂→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(III) in ferritin. Fe₂O₃(H₂O) has paramagnetism.
    These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.

2.Materials!&!Methods

2.1.Parts

2.2.Expression check

SDS pageについて書く

2.3.鉄耐性の評価

2.4.磁性評価

3.Results & Discussion

3.1.プラスミド

3.2鉄耐性の評価

3.3磁性評価

4.Conclusion

それは・・