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BostonU iGEM Team: Welcome
Our Team
Team
Summer Fun
Project
Project Overview and Abstract
Introduction to MoClo and Characterization
Quorum Sensing
Histidine Kinase
Data Sheet App
Methodology Overview
Results Summary
Achievements
Data Collected
Parts Submitted
MoClo Kit
Medal Fulfillment
Notebook
Protocols
Characterization Notebook
Quorum Sensing Notebook
Histidine Kinase Notebook
Clotho Notebook
Puppeteer Notebook
Troubleshooting
Considerations
New England iGEM Regional Meeting
Human Practices
Safety
Acknowledgements
Collaborations
Acknowledgements
Protocols
This section contains the protocols that we used during the summer and that are mentioned in our Notebook. We hope they help anyone interested in our experiments to successfully repeat them.
Phusion PCR
Transformation with Gold Bioline Cells
Restriction Digest
Gel Extraction
Miniprep
DNA Quantification
Media Prep
Ligation