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Cornell University Genetically Engineered Machines
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Plasmids
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2013: Organofoam
Team Website
2012: SAFE BET
2011: Biofactory
2010: OMG OMVs
2009: Cadmium Sensor
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DNA Purification
EZNA Gel Extraction
EZNA Miniprep
Qiagen Gel Extraction
Qiagen Miniprep
DNA Assessment
DNA Gel Electrophoresis
Preparation of DNA for Sequencing
PCR Methods
Phusion PCR
Taq PCR
Vent PCR
Plasmid Insertion
Electroporation of
E.coli
Preparing Electrocompetent
E.coli
Myers and Myers Electoporation of
Shewanella
PNNL’s Preparing Electrocompetent
Shewanella
Conjugation of Plasmids into
Shewanella
Plasmid Editing
Double Digest
Single Digest
Site Directed Mutagenesis
T4 Ligase Ligation
Shewanella
Growth
Shewanella
Growth Assay Protocol
M4 Recipe
Synthetic River Media Recipe
Plate Drying