Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.10RNasePreparation

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Phage Purification May - June Notebook: Experiments

6.10 RNase A Preparation

I) Purpose

Prepare RNase to be used in phage purification procedures.

II) Expected Outcome

Viable stock of RNase.

III) Reagants Used

Sigma-Aldrich Ribonuclease A from bovine pancreas (powder form)

10 mM sodium acetate buffer, pH 5.2

1 M Tris-HCl, pH 7.4

IV) Actual Procedure

Add 10 mL of 10 mM sodium acetate buffer to the RNase powder.

Heat to 100^◦ C for 15 minutes.

Allow solution to cool to room temperature.

Adjust to pH 7.4 using 1 M Tris-HCl.

Aliquot and store in -20^◦ C.

V) Results

We created an RNase A solution that can be used in the phage purification procedure.