Team:MIT/ResultsBiobricks
Overview
siRNA Characterization
Exosome Isolation and Co-Culturing
Cell-Cell Co-Culturing
Overview
GFP
rtTA3
Cre
L7Ae
Cas9-VP16
Overview
Leucine Zipper Fusion
DNA Sensing
Biobricks
Favorite Parts:
Name: CMV_Acyl-TyA-eGFP MammoBlock
ID: BBa_K1179071
Type: Generator
Length: 2834
Description/Usage: This part encodes the expression of green fluorescent protein that is exported out of the cell from the addition of the acylation tag and TyA sequence that is fused to the GFP coding sequence. ID: BBa_K1179002
Type: Generator
Length: 5141
Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with Cas9 binding sites upstream. ID: BBa_K1179072
Type: Generator
Length: 2085
Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.
Acyl-TyA
BBa_K1179000
Acyl-TyA-eGFP
BBa_K1179001
Hef1a_Cas9-VP16
BBa_K1179002
Acyl-TyA-mKate-His
BBa_K1179003
Acyl-TyA BBa_K1179000
Acyl-TyA-eGFP BBa_K1179001
Hef1a_Cas9-VP16 BBa_K1179002
Acyl-TyA-mKate-His BBa_K1179003
Acyl-TyA-rtTA BBa_K1179004
Acyl-TyA-rtTA-His BBa_K1179005
Acyl-TyA-Tev BBa_K1179006
Acyl-TyA-TEV-rtTA-His BBa_K1179007
Cas9 D10A & H840A mutation-linker BBa_K1179008
Cas9-Linker-VP16 BBa_K1179009
eBFP BBa_K1179010
eYFP BBa_K1179012
eYFP-PEST BBa_K1179013
VP16-GAL4 BBa_K1179014
U6_gRNA (Cr9) BBa_K1179015
L7Ae BBa_K1179016
Linker-Cas9 D10A & H840A mutation BBa_K1179017
mKate –PEST BBa_K1179018
mKate-His BBa_K1179019
rtTA-His BBa_K1179020
CAG_Acyl-TyA-mKate-His BBa_K1179021
CAG_Acyl-TyA-rtTA BBa_K1179022
CAG_Acyl-TyA-rtTA-His BBa_K1179023
CAG_eYFP-PEST BBa_K1179024
CAG_L7Ae BBa_K1179025
CAG_mKate-PEST BBa_K1179026
CAG-tagBFP BBa_K1179027
D10A and H840A mutant Cas9-VC155 BBa_K1179028
D10A and H840A mutant Cas9-VN155 BBa_K1179029
D10A and H840A mutant Cas9-VN173 BBa_K1179030
CMV_ L7Ae_myc-His6 BBa_K1179032
CMV_2xKMet-eGFP BBa_K1179033
L7Ae-2a-eYFP BBa_K1179037
TRE_Cas9-VP16 BBa_K1179040
U6_gRNA 1A Reverse BBa_K1179041
U6_gRNA 1A BBa_K1179042
U6_gRNA 2A BBa_K1179043
U6_gRNA 3A BBa_K1179044
U6_gRNA 4A BBa_K1179045
U6_gRNA 5A BBa_K1179046
U6_gRNA 6A BBa_K1179047
U6_gRNA 6A Reverse BBa_K1179048
VC155 BBa_K1179049
VC155 with Leucine zipper BBa_K1179050
VC155-Cas9(D10A and H840A mutant) BBa_K1179051
VN155 BBa_K1179052
VN155 with Leucine zipper BBa_K1179053
VN155-Cas9(D10A and H840A mutant) BBa_K1179054
VN173 BBa_K1179055
VN173 with Leucine zipper BBa_K1179056
VN173-Cas9(D10A and H840A mutant) BBa_K1179057
Cre entry vector BBa_K1179058
eYFP-4x451ts BBa_K1179060
eYFP-4x503ts BBa_K1179061
LacI BBa_K1179062
LacI-4x451ts BBa_K1179063
LacI-4x503ts BBa_K1179064
TagBFP BBa_K1179068
CAG BBa_K1179069
CMV_Acyl-TyA-eGFP BBa_K1179071
Hef1a_eYFP-4x451 BBa_K1179072
TRE-tight_eYFP-4x451 BBa_K1179073
Hef1a_eYFP-4x503 BBa_K1179074
TRE-t_eYFP-4x503 BBa_K1179075
"
