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Team:DTU-Denmark/Notebook/17 September 2013
From 2013.igem.org
Contents |
Description
The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anerobically. Specifically, we measure nitrous oxide production over a period of 10 hours.
Methods
Protocol Bioreactor
Results
Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment.
| Time | Replicate 1 | Replicate 2 | Replicate 3 | Control: Distilled Water |
|---|---|---|---|---|
| 14:00 | 1.84 mg/L | 1.64 mg/L | 1.60 mg/L | N/A |
| 22:00 | 1.43 mg/L | 1.44 mg/L | 1.50 mg/L | 1.65 mg/L |
Discussion
There were several sources of error in this experiment. Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors.
