"
Team:IIT Madras/Notebook
From 2013.igem.org
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Phase I : Ideation
Phase II : Experimentation
e-Notebook
| May 13 | June 13 | July 13 | ||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| S | M | T | W | T | F | S | S | M | T | W | T | F | S | S | M | T | W | T | F | S | ||||||||||||||
| 1 | 2 | 3 | 4 | 1 | 1 | 2 | 3 | 4 | 5 | 6 | ||||||||||||||||||||||||
| 5 | 6 | 7 | 8 | 9 | 10 | 11 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | ||||||||||||||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | ||||||||||||||
| 13 | 14 | 15 | 16 | 17 | 18 | 19 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | ||||||||||||||
| 20 | 21 | 22 | 23 | 24 | 25 | 26 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 28 | 29 | 30 | 31 | |||||||||||||||||
| 27 | 28 | 29 | 30 | 31 | 30 | |||||||||||||||||||||||||||||
Experimental results
Protocol
1. Transformation:
(a) Added 1-2µl of DNA to competent cell.
(b) Kept on ice for 30 minutes.
(c) Heat Shock was given at 42°C for 90 sec.
(d) Kept on ice for 5 min.
(e) Added 400µl of LB media.
(f) Incubated at 37°C for 1h in shaker.
(g) Plating was done with 250µl of culture.
(h) Plates were incubated overnight at 37°C.
Phase III
Troubleshooting
Discussions
This page is under construction. For more information contact Rohan or Aman.
