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Team:SDU-Denmark/Tour41
From 2013.igem.org
Complete Journal
Important dates & Protocols
"If it is not documented, it doesn’t exist." - Louis Fried
Obviously, Louis Fried was wrong - and luckily so, we might add! But it is true that if it is not documented, we don't know whether it exist. So please, click through the tabs below and catch a quick glimpse of our most treasured moments of the summer. We kept a calender of special events since the start of the project. And if you have the yearning (particularly after reading our Results), open up our thorough protocols below.
08.03.2013 - 10.03.2013 - Teambuilding: iGEM crash course (DTU)
21.03.2013 - First meeting: get to know each other
26.03.2013 - Two new members of the team (Mattias and Sofie)
April + start of May - Brainstorming
08.05.2013 - Determination of project
11.07.2013 - One new member of the group (Nicky)
25.07.2013 - B.sub DXS biobrick
25.07.2013 - E.coli IspG biobrick
29.07.2013 - pSB1C3-Plac-dxs(B. sub)-linker-GFP
31.07.2013 - pSB1C3-Plac-dxs(E. coli)-linker-GFP
01.08.2013 - At this point we realized we should overexpress lacI (lac repressor) in order to see a repression
01.08.2013 - Sequencing of E. coli dxs standard part
05.08.2013 - pSB1C3-Plac-dxs(B. sub)
19.08.2013 - HRT2 biobrick
19.08.2013 - pSB1C3-Plac-dxs(E. coli)
19.08.2013 - pSB1C3-Pcon-lacI:LVA-term
17.09.2013 - LacI BioBrick
17.08.2013 - pSB1C3-Pcon-araC-term
21.08.2013 - pSB1C3-lacI:LVA-Plac-dxs(B. sub)
23.08.2013 - pSB1C3-lacI:LVA-Plac-dxs(B. sub)-linker-GFP
24.08.2013 - Characterization of lacI:LVA function
24.08.2013 - Confirmation that we could control the expression of Dxs with lacI:LVA using IPTG
28.08.2013 - pSB1C3-lacI:LVA-Plac-dxs(E. coli)
30.08.2013 - pSB1C3-Para-HRT2-(FLAG)
30.08.2013 - Growth experiment for MG1655/pSB1C3-lacI:LVA-Plac-dxs(B.sub)+/-GFP
13.09.2013 - pSB1C3-araC-Para-HRT2-(FLAG)
17.09.2013 - pSB1C3-lacI-Plac-dxs(B. sub)-linker-GFP
17.09.2013 - Characterized device with lacI and found that it functions better than lacI:LVA
19.09.2013 / 22.09.2013 - pSB1C3-lacI-Plac-dxs(B. sub)
20.09.2013 - Growth experiment for MG1655/pSB1C3-lacI-Plac-dxs(B.sub)
21.09.2013 - pSB1K3-araC-Para-HRT2-(FLAG)
21.09.2013 - Growth experiment for MG1655/pSB1C3-lacI-Plac-dxs(B.sub)-GFP
30.08.2013 - Track selection
30.08.2013 - Project title
23.09.2013 - BioBricks Part DNA shipped to iGEM HQ
25.09.2013 - BioBricks Part DNA at the Registry (Received at iGEM)
04.10.2013 - Wiki freeze
11.10.2013 - 13.10.2013 - Regional Jamboree
When working together in a large group, particularly on such a large project, it is simply impossible for anyone to maintain an overview over the entire project in real time. That is why it is important to keep protocols of every part of the project, to document every experiment that is performed. This allows each team member to backtrack and to resolve any issues with (or mistakes made in) previous experiments. Countless digestions and hundreds of PCR reaction were done, and the explicit protocols established which setups were effective, and which were not. Our iGEM-team has kept protocols since the dawn of the project, and has proved to be an invaluable resource, which saved us quite a bit of time.
Click on the project title below to expand the window and get an overview of the protocols for each project part. Each protocol name is a link to a pdf file that will open in a new window.
