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Sensing

PCR of the BBa_J23119 part from iGEM
In order to have a positive control for our sensing constructs we wanted to use a constitutive promoter that would express the superfolded GFP all the time. For this we used a promoter from iGEM which we inserted instead of the arabinose sensitive promoter into the pSB1C3 plasmid.
The PCR of this promoter worked very well.